Phospho foxo1
WebAug 2, 2005 · All reaction products were analyzed by Western blotting using anti-phospho-Foxo1 antibody or silver stain. Discussion. Our present study provides two findings about the consequences of Foxo1 acetylation, the attenuation of DNA-binding ability and the enhancement of the level of Foxo1 phosphorylation. We further explored the relevance of … WebJan 25, 2024 · Total protein lysates were analyzed by western blot with anti-phospho-FOXO1 (phospho-Thr24, phospho-Ser256 and phospho-Ser319), anti-FOXO1 and anti-β-actin antibodies. (B) HEK293T cells were ...
Phospho foxo1
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WebSep 1, 2004 · The membranes were blocked with 5% nonfat dry milk, Tris-buffered saline, and 0.05% Tween 20 for 1 h at room temperature and then probed with anti-FoxO1 (Cell Signaling Technology, Beverly, MA) to detect FoxO1, anti-phospho-FoxO1 (Ser-256, Cell Signaling Technology) to detect phosphorylated FoxO1, and anti-α-tubulin (ICN) as an … WebSeveral transcription factors, including NF-κB, AP-1, and FoxO1, are known to mediate the induction of MMP-9 expression following stimulation by different factors and in various types of cells. 1,25,26,31,32 In our previous study, we also showed that NF-κB and AP-1 contribute to LPS-induced MMP-9 expression in RBA-1 cells. 12,33 Here, we ...
WebSep 1, 2005 · FoxO1 translocates to the nucleus in response to H 2 O 2. A) We analyzed FoxO1 phosphorylation by Western blotting with antisera against phospho-Ser256, phospho-Ser316, and anti-FoxO1. B) Immunocytochemical analysis of FoxO1 distribution in βTC-3 … WebNov 25, 2015 · # p values of <0.05 indicate significant differences from treatments without UP; (D) The levels of phospho-FOXO1 and phospho-p70S6K1 were measured after treatment with Akt inhibitor by Western blotting. Tubulin was detected as a loading control.
WebFOXO1 (Phospho-Ser256) DNA-Binding ELISA Kit. Detection Target: FOXO1 (Phospho-Ser256) Detection Range: 10-30 ug of total protein/well. Quantity: 96 Tests. Supplier Page. Compare Product. WebPhospho-Fox01 (Ser256) Antibody detects endogenous levels of Fox01 only when phosphorylated at serine 256. The antibody cross-reacts with Fox04 phosphorylated at Ser193. Species Reactivity: Human, Mouse, Rat, Monkey Species predicted to react based …
WebFOXO1 is involved in the insulin regulation of glucose-6-phosphatase (G6p) expression. Additionally, this protein plays a vital role in tumor suppression by inducing growth arrest and apoptosis. Features and Benefits Evaluate our antibodies with complete peace of mind. dan ritchey ohioWebPhospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody detects exogenous levels of FoxO1 only when phosphorylated at serine 319 and exogenous levels of FoxO4 only when phosphorylated at serine 262. The antibody does not cross-react with FoxO1 phosphorylated at other sites, FoxO4 phosphorylated at other sites nor with FoxO3a phosphorylated at any … birthday party ideas 42WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). The Tunel assay … birthday party ideas alWebPhospho-FoxO1 (Ser256) (E1F7T) recognizes endogenous levels of FoxO1 protein only when phosphorylated at Ser256. The antibody cross-reacts with overexpressed FoxO4 phosphorylated at Ser193 and may cross-react with overexpressed FoxO3a phosphorylated at Ser253. The antibody also cross-reacts with a protein of unknown origin around 160kD. birthday party ideas bromleyWebMay 2, 2016 · Recent data indicate that ~9% of DLBCLs harbor FOXO1 mutations that augment its nuclear localization due to the loss of 14-3-3 chaperone-binding and are associated with inferior outcome after R ... birthday party ideas atlantaWebProduct name Anti-FOXO1A (phospho S256) antibody See all FOXO1A primary antibodies Description Rabbit polyclonal to FOXO1A (phospho S256) Host species Rabbit Tested applications Suitable for: WB, IHC-P, ICC/IF more details Species reactivity Reacts with: … dan river ashley sheetsWebPhospho-FoxO1 (Ser256) UniProt ID: Q12778: NCBI: 2308: Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Description: FoxO1 (phospho-Ser256) antibody (FITC) Alternative names: birthday party ideas boise idaho