G6pd shrna
WebNational Center for Biotechnology Information WebCatalog #. AM16708. Standard 5 nmol. Purification: HPLC In-Vivo Ready Standard. Size: 5 nmol 20 nmol 20 nmol 20 nmol 20 nmol 40 nmol 40 nmol 100 nmol 250 nmol 250 …
G6pd shrna
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WebApr 9, 2024 · This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many … WebJan 29, 2024 · AGS cells co-transfected with lentivirus-mediated sh-G6PD#1 and sh-G6PD#2 or shRNA-control were subcutaneous injected into the back of nude mice respectively. The results of PCR assay indicated that G6PD expression level was significantly down-regulated in sh- G6PD #1 and sh- G6PD #2 group compared with …
WebJul 10, 2024 · Delivery of G6PD shRNA with poly (amino-co-ester) polyplexes enhances oxaliplatin efficacy in PDX models. PDX tumors have been suggested as a more realistic experimental and preclinical model. 21 In addition to cell line-based xenograft models, PDX models were also performed to assess clinical benefits of targeting G6PD. WebFeb 20, 2011 · The target sequence for G6PD shRNA #1 (Catalogue No. RHS3979-9595543) is 5′-GTCGTCCTCTATGTGGAGAAT-3′, and the target sequence for G6PD …
WebNov 15, 2024 · To induce Plk1 and G6PD shRNA expression, cells were grown in the presence of 0.1 μg/ml Dox (Sigma). Lenti-viral plasmids pSin-Plk1 and pSin-G6PD (WT and mutants), were co-transfected with ... WebJan 3, 2024 · G6PD Antibody (G-12) is an IgG 1 κ mouse monoclonal G6PD antibody (also designated Glucose-6-Phosphate 1-Dehydrogenase antibody, or G6PD1 antibody) that detects the G6PD protein of human origin by WB, IP, IF, IHC(P) and ELISA. G6PD Antibody (G-12) is available as both the non-conjugated anti-G6PD antibody form, as well as …
WebJun 25, 2024 · shRNA 序列 与 G6PD siRNA 基因沉默序列相对应; 转染后，可以用嘌呤霉素筛选得到稳定表达 shRNA 的细胞; 生物安全信息－慢病毒颗粒没有自我复制功能，当 …
WebJul 23, 2024 · After 48 h of cell transfection using the mixture, fresh medium was replaced. Sequences of transfection plasmids were as follows: Human ALKBH5 shRNA #1: TCGTGTCCGTGTCCTTCTT; ALKBH5 shRNA #2: GACTGTGCTCAGTGGATAT; Human G6PD shRNA #1: GGGCTATTTCGATGAATTTGG; G6PD shRNA #2: … chemistry elements and symbols worksheetsWebTumor cells often lose the balance of oxidation and antioxidation, but the role of G6PD in such an imbalance is still largely unknown. To investigate the related function of G6PD in … flight from iah to lagos nigeriaWebThe G6PD RNAi-1 shRNA targeting sequence is 5′-GGGCTATTTCGATGAATTTGG-3′, the G6PD RNAi-2 shRNA targeting sequence is 5′-AACTCAGATGACGTCCGTGAT-3′, and the control shRNA sequence is 5′-CCGCAGGTATGCACGCGT-3′. All the shRNA was synthesized by HonorGene (Changsha, China). Reverse Transcription‑quantitative PCR … chemistry element tableWebJul 10, 2024 · To test this hypothesis, G6PD was stably knocked down in DLD-1 and HCT116 cells which exhibited high expression levels of … chemistry elements namesWebSep 22, 2006 · The shRNA expression cassettes in pHyper1G101 and pHyper1G102 encode G6PD shRNA sh101 and scrambled control shRNA sh102, respectively. The sh101 targets nucleotide 137–156 of G6PD mRNA. The target recognition sequence of sh102 expression cassette has the same base composition but in a randomized order. chemistry elements symbolsWebJan 9, 2024 · The mini-cassette encoding shRNA against G6PD, p22 phox, or scramble gene include overhangs that are allowed cloning into pLKO.1.null-T. Scramble shRNA was used as a negative control. The sequence ... chemistry elephant toothpaste experimentWebTumor cells often lose the balance of oxidation and antioxidation, but the role of G6PD in such an imbalance is still largely unknown. To investigate the related function of G6PD in tumor cells, we established a stable line of A375 human melanoma cells with G6PD gene knockdown by a shRNA lentiviral cloning and expression system. flight from iah to miami