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Web1 day ago · Strengthening livestock systems through science and innovation. The Animal Reproduction and Biotechnology Center will be a further collaboration between the … WebOct 22, 2024 · The pGL3.0-DNMT1 (-1000–0 bp) reporter gene plasmids (DNMT1 promoter WT and Mutant) were constructed by Biogot technology Company. The detailed protocol and the DNMT1 promoter WT and Mutant DNA sequences (-1000–0 bp) are shown in Additional file 1. Animal works of tumour xenograft model and lung-colonization … canandaigua daily messenger newspaper
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WebNov 11, 2024 · Myc-Ub and Myc-UbK48R expression constructs were obtained from Biogot technology (Nanjing, Jiangsu, China). HEK293T cells were transfected with the above plasmids using polyethylenimine (PEI) in Opti-MEM medium (Invitrogen). Co-immunoprecipitation (Co-IP) assay. ... (Cell Signaling Technology), followed by … Webchased from Biogot Technology) embarked the sequences of TRIP13 or SP1. The stable cells were obtained by selecting with puromycin (2 μg/mL) for 7 days. Dual-Luciferase Reporter Assay The mutant TRIP13 promoter was constructed by point mutation—the binding sites' sequence was Webphospho(p)-ERK antibodies were purchased from Biogot Technology (Nanjing, China). The VEGFR antibody was obtained from Affinity Biosciences (Cincinnati, OH, USA). All other chemicals and reagents were of analytical grade and obtained from local chemical companies. Composition and Preparation of QD canandaigua coffee shop